Different bacteriophage systems can be utilized for phage display, including the T4, lambda, as well as the filamentous M13 bacteriophage. One approach is phage display selection, which is a robust, easy-to-perform, and inexpensive method by which specific antigen binders are selected from large combinatorial libraries containing billions of antibody fragments.Īs antibody phage display is gaining increasing interest in the field of toxinology, the intention with this review is to provide both basic and more advanced knowledge on the underlying science behind the technology and the lifecycle of the M13 phage.Ĭentral to phage display technology is the biology of the bacteriophage used to display antibodies. To discover and develop antibodies, different techniques can be harnessed. Moreover, these therapeutic proteins could be manufactured cost-competitively using modern cell cultivation methods employed for large scale production. One promising approach seems to be the use of human IgG antibodies and/or camelid antibody fragments, as these molecules can be used to develop recombinant antivenoms with high efficacy and safety due to their compatibility with the human immune system. Several different avenues aimed at bringing innovation into the field of snakebite antivenoms have been pursued, including medicinal chemistry approaches, novel immunization techniques, and the use of biotechnological strategies. This creates renewed hope for snakebite victims worldwide and could potentially lead to a mobilization of scientific efforts toward the development of novel snakebite envenoming therapies. A continuous, concentrated stream of product is harvested from the bioreactor over several weeks to months.Ĭell Culture Company can effectively scale up the clone producing antibodies and expand the production of the hybridoma cell line for clients.Ĭontact us today for inquiries regarding our unique process for monoclonal antibody production.With the recent inclusion of snakebite envenoming on the World Health Organization’s list of Neglected Tropical Diseases, focus on both prevention and treatment of this infliction has increased. This bioreactor environment is monitored and maintained at ideal conditions for antibody production.
At Cell Culture Company we offer an alternative, easy-to-scale, perfusion-based bioreactor approach to monoclonal antibody production.Ĭells are cultured to inoculate a single-use hollow fiber cartridge, in which a consistent flow of media delivers nutrients across the semi-permeable fibers. Standard Monoclonal Antibody Production ProcessĪ traditional monoclonal antibody manufacturing process involves immunizing mice, producing hybridomas, screening the clones, and scaling up the clone production. Our team of scientists provide antibodies tailored to customer specification.
The monoclonal antibodies produced at Cell Culture Company have been used as reagents for research, diagnostics, and product development.Ĭell Culture Company offers hybridoma monoclonal antibody production services, in addition to accepting clients’ other cell lines for antibody production. Designing and generating custom monoclonal antibodies has proven particularly helpful in treating certain types of cancer.
Monoclonal antibody (mAb) production is critical to the research and treatment of many infectious and non-infectious diseases. Our highly experienced team has the capability and expertise to perform large-scale antibody production.Ĭell Culture Company offers a wide range of services, including purification and downstream processing for monoclonal antibodies. We have produced thousands of custom monoclonal antibodies with high specificity and high affinity. Contact Us About Monoclonal Antibody ProductionĪt Cell Culture Company, we are fully equipped to provide custom monoclonal antibody production services.
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